Lipidomics of intact mitochondria by MALDI-TOF/MS

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Lipidomics of intact mitochondria by MALDI-TOF/MS.

A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly applied over the target and, after drying, covered by a thin layer of the 9-aminoacridine matrix solu...

متن کامل

Screening of nerve agent degradation products by MALDI-TOFMS.

A novel method for the rapid screening of degradation products derived from nerve agents by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is described. Five standard products were selected as model compounds, including isopropyl methylphosphonic acid (IMPA), pinacolyl methylphosphonic acid (PMPA), ethyl methylphosphonic acid (EMPA), isobutyl methylphosphonic acid ...

متن کامل

Development of a multi-frequency laser for use in MALDI-TOFMS.

The application of a multi-frequency laser source for the use in matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described. An elliptically polarized beam of a Nd:YAG laser emitting at 355 nm (200 mJ) is focused into a Raman shifter, filled with high pressure hydrogen. As a result, numerous Raman lasers (including vibrational and rotational Raman em...

متن کامل

Method for estimating the isotopic distributions of metabolically labeled proteins by MALDI-TOFMS: application to NMR samples.

We have developed an efficient method of estimating metabolic incorporation of heavy isotopes into proteins, including those where a single amino acid carries the label. The protein is digested with trypsin, and the resulting peptide mixture is examined directly by MALDI-TOF mass spectrometry. Peptides are chosen for analysis if they contain one or more labeled atoms and also exhibit clearly se...

متن کامل

Metabolism of S-nitrosoglutathione in intact mitochondria.

S-nitrosation of protein thiol groups by nitric oxide (NO*) is a widely recognized protein modification. Only few intracellular S-nitrosated proteins have been identified and it has been reported that S-nitrosation/denitrosation can serve as a regulatory process in signal-transduction pathways. Given the potential physiological importance of S-nitrosothiols, and considering that mitochondria ar...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 2012

ISSN: 0022-2275

DOI: 10.1194/jlr.d026203